An Improved Medium for the In VitroRooting of Harbrite Peach

Abstract

Shoots derived from proliferating cultures of Harbrite peach and Stanley plum can be induced to form multiple roots and extensive shoot growth on a new rooting medium (MS-H). The MS-H medium is composed of Murashige and Skoog high mineral salts with (amounts per liter) myo-inositol (200 mg), casein hydrolysate (1000 mg), glycine (2.0 mg), Biotin (0.05 mg), thiamine-HCl (2.0 mg), choline chloride (1.0 mg), nicotinic acid (2.5 mg), pyridoxine-HCl (0.25 mg), para-aminobenzoic acid (1.0 mg), folic acid (0.25 mg), pantothenic acid (0.5 mg), indolebutyric acid (3.0 mg), naphthaleneacetic acid (1.0 mg), gibberellic acid (GA ₃) (0.1 mg), kinetin (0.04 mg), 6-benzylaminopurine (0.01 mg), sucrose (20 g), and agar (6 g). Rooted shoots have been successfully transfered to soil.