Induced Mutations in Citrus Shoot Tip Culture in Vitro
DOI:
https://doi.org/10.71318/apom.1985.39.2.38Abstract
This study was undertaken to determine the efficacy of using induced mutation of the callus of citrus clones as a method in citrus breeding. It was observed that the nucellar clones had a higher ratio of callus cell differentiation than the old clones. The callus cells were exposed to gamma irradiation of 7,000 roentgen at 127 R/min.
Callus cells derived from the shoot tip proved to be more appropriate for cytological examination by the smearing technique than the root tip cells. Gamma irradiation of callus cells is quite effective for inducing chromosome aberration and micronucleus inspection.
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