Culture Date and Germination Procedure Affects Success of Nectarine Ovule and Embryo Culture
DOI:
https://doi.org/10.71318/apom.1994.48.3.173Abstract
Immature ‘Sunlite’ nectarine ovules were grown on Knop’s ovule growth medium for 30 days and germinated following stratification either in vitro on a modified Knop’s germination medium or in moist perlite. The minimum age at which any embryos were successfully ovule cultured and germinated was 53 days after full bloom. Germination percentage increased significantly at 60 and 67 days after full bloom. Germination percentage in perlite was not greatly different from that of ovules cultured in vitro on modified Knop’s germination medium, but the 53 and 60 days after full bloom embryos germinated in perlite were visibly weaker.
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