Liquid Medium Overlays Enhance Growth and Multiplication of In VitroGrapes and Apples
DOI:
https://doi.org/10.71318/apom.1996.50.2.86Abstract
Liquid medium overlays of established cultures were compared to transfers to fresh medium. The number of micropropagated grape ( Vitissp.) shoots increased 67%, shoot length 73%, and fresh weight 125% with liquid medium overlays as compared to transfer to fresh medium. Callus growth at the base of shoots was 64% less. In apples ( Malus pumila), the number of micropropagated shoots increased 35%, shoot length 69%, and fresh weight 67% with liquid medium overlays. Callus fresh weight decreased by 52% when compared to cultures transferred to fresh solid media.
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