New Tetraploid Breeding Parents for Triploid Seedless Citrus Cultivar Development
DOI:
https://doi.org/10.71318/apom.1996.50.2.76Abstract
Protoplast culture following polyethylene glycol (PEG)-induced fusion resulted in the regeneration of somatic hybrid plants from ‘Succari’ sweet orange ( C. sinensisL. Osbeck) with ‘Dancy’ tangerine ( C. reticulataBlanco), ‘Minneola’ tangelo ( C. paradisiMacf. X C. reticulataBlanco), ‘Murcott’ tangor (purported C. reticulatax C. sinensis), ‘Page’ tangelo [‘Minneola’ tangelo x ‘Clementine’ mandarin ( C. reticulata)], and ‘Ponkan’ mandarin ( C. reticulata). ‘Succari’ protoplasts were isolated from ovulederived embryogenic cell suspension cultures, and protoplasts of the mandarin-type parents were isolated from seedling leaves. Somatic hybrid plants were identified on the basis of leaf morphology, root tip chromosome number, and isozyme and RAPD analyses. These tetraploid plants may have direct cultivar potential; however, their greatest value will be as pollen parents to be crossed with selected monoembryonic diploids to produce seedless triploids.
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