DNA Markers Based on PSYGenes Can Differentiate Yellow- and White-Fleshed Loquats

Abstract

White-flesh is one of the most appreciated traits in loquat. The development of new white-fleshed cultivars is costly and time consuming, involving the generation and selection of thousands of progeny each year. Marker-assisted selection (MAS) is an excellent technique to accelerate conventional breeding approaches. In this study, a specific DNA marker based on the sequence of phytoene synthase ( PSY) gene was identified as an ideal DNA marker for the identification of fruit flesh color. We screened 31 cultivars derived from different countries and 24 F ₁hybrids developed from 4 cross combinations, and analyzed the relationships between flesh color and the DNA marker. The results showed that the DNA marker not only can distinguish yellow- and white-fleshed cultivars accurately, but can be used for accurate and early selection of yellow and white flesh progeny derived from controlled hybridizations.